K. Woodward et al., REGIONAL LOCALIZATION OF 64 COSMID CONTIGS, INCLUDING 18 GENES AND 14MARKERS, TO INTERVALS ON HUMAN-CHROMOSOME 9Q34, Genomics, 29(1), 1995, pp. 257-260
A fluorescence in situ hybridization map of distal human chromosome 9q
has been produced by mapping cosmid clones to metaphase chromosomes w
ith balanced reciprocal translocations. This is a very accurate method
of mapping, as clones are localized by their position with respect to
the breakpoint in addition to cytogenetic banding. By using three lym
phoblastoid cell lines with translocation breakpoints within 9q34, we
have localized 18 genes and 14 DNA markers to one of four intervals on
the chromosome. Cosmid contigs exist around 16 of these genes and 12
of these markers. A further 43 contigs have also been mapped, but they
are as yet anonymous. (C) 1995 Academic Press, Inc.