ORGANIZATION AND CHROMOSOMAL LOCALIZATION OF THE GENE (TAF2H) ENCODING THE HUMAN TBP-ASSOCIATED FACTOR-II-30 (TAF(II)30)

The basal RNA polymerase II transcription factor, TFIID, is composed o f the TATA binding protein (TBP) and 8-13 TBP-associated factors (TAFs ) ranging from 250 to 17 kDa. The structure of the human gene encoding the 30-kDa subunit of TFIID, TAF2H, has been determined. The gene con sists of five exons (ranging from 66 to 248 bp) and four introns (rang ing from 83 to 211 bp). The transcription start site of the mRNA was m apped, and it shares a weak homology to the consensus of known initiat or elements. Using in situ hybridization on human metaphase chromosome s, the TAF2H gene has been localized in the 11p15.2-p15.5 region of th e human genome. (C) 1995 Academic Press, Inc.