ZEON-1, A MEMBER OF A NEW MAIZE RETROTRANSPOSON FAMILY

Citation
Wm. Hu et al., ZEON-1, A MEMBER OF A NEW MAIZE RETROTRANSPOSON FAMILY, MGG. Molecular & general genetics, 248(4), 1995, pp. 471-480
Citations number
53
Categorie Soggetti
Genetics & Heredity",Biology
ISSN journal
00268925
Volume
248
Issue
4
Year of publication
1995
Pages
471 - 480
Database
ISI
SICI code
0026-8925(1995)248:4<471:ZAMOAN>2.0.ZU;2-2
Abstract
We have previously shown that the tandemly duplicated 27 kDa maize sto rage protein locus underwent mitotic rearrangement to yield a single-c opy allele in isolates of the inbred line A188. This rearrangement con tains a new LTR retrotransposon, designated Zeon-1. This middle repeti tive element of 7313 bp had two long terminal repeats, a primer bindin g site, a polypurine tract and a gag-related open reading frame of 375 amino acids. Transcripts of the gag-related region were detected by t he polymerase chain reaction (PCR) in certain maize tissues, and Weste rn blots detected the gag-related protein in the same tissues. Moreove r, the product of this mitotic rearrangement was shown to contain the same insertion site and 3' LTR as Zeon-1, suggesting that this rearran gement occurs with unusual precision. Zeon elements were found to be p resent in teosinte and not present in the Gramineae wheat, barley, sor ghum and rye.