Jt. Vanoirschot et al., VIRULENCE AND GENOTYPE OF A BOVINE HERPESVIRUS-1 ISOLATE FROM SEMEN OF A SUBCLINICALLY INFECTED BULL, Veterinary record, 137(10), 1995, pp. 235-239
A bovine herpesvirus 1 (BHV-1) isolate from the semen of a subclinical
ly infected bull was administered to cattle by various routes to asses
s its virulence, Cattle that were artificially inseminated or inoculat
ed intrapreputially did not develop clinical signs, but did transmit t
he virus to contact cattle, However, the isolate induced severe signs
of rhinotracheitis and vulvovaginitis in cattle that were inoculated b
y the intravaginal, intranasal or intravenous routes, but did not infe
ct the fetus, The isolate was therefore not of low virulence, Analysis
with DNA restriction enzymes could not assign the isolate to either t
he BHV-1 . 1 Or BHV-1 . 2 genotype.