ONLINE COUPLING OF FLOW FIELD-FLOW FRACTIONATION AND MULTIANGLE LASER-LIGHT SCATTERING FOR THE CHARACTERIZATION OF POLYSTYRENE PARTICLES

The on-line coupling of a now held-how fractionator (F-4) and a multia ngle laser light scattering detector (MALLS) produces a novel analytic al system for dispersed samples, Absolute distribution measurements ar e obtained for the molar mass and radius of gyration, which is directl y related to the particle diameter, In this work, polystyrene latex st andards (50, 105, and 304 nm and a mixture) dispersed in water were us ed to investigate the accuracy of a system with on-line coupling of F- 4 and MALLS, Tests were carried out in a constant field of force and f or the first time in a programmed field of force mode, The results sho w that it is possible to use MALLS and a decreasing cross-flow for sep aration and that, even with changing cross-flow and under nonideal elu tion conditions, coupling to MALLS gives more reliable results than th ose obtained with a F-4 system alone.