CONTRIBUTION OF THE INDIVIDUAL COMPONENTS OF THE DELTA-ENDOTOXIN CRYSTAL TO THE MOSQUITOCIDAL ACTIVITY OF BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS

Citation
N. Crickmore et al., CONTRIBUTION OF THE INDIVIDUAL COMPONENTS OF THE DELTA-ENDOTOXIN CRYSTAL TO THE MOSQUITOCIDAL ACTIVITY OF BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS, FEMS microbiology letters, 131(3), 1995, pp. 249-254
Citations number
20
Categorie Soggetti
Microbiology
Journal title
ISSN journal
03781097
Volume
131
Issue
3
Year of publication
1995
Pages
249 - 254
Database
ISI
SICI code
0378-1097(1995)131:3<249:COTICO>2.0.ZU;2-5
Abstract
The delta-endotoxin crystal of the mosquitocidal bacterium Bacillus th uringiensis subsp. israelensis contains four major delta-endotoxins. E xpression systems were devised to synthesize each of the four toxins a t concentrations at which they formed inclusion bodies in an acrystall iferous mutant of Bacillus thuringiensis. The relative activities of t hese inclusions were then determined against Aedes aegypti larvae. Bio assays of mixtures of the individual toxins revealed a number of syner gistic interactions which explained in part why the native crystal is considerably more toxic than any of the individual toxins.