STRUCTURALLY IDENTICAL PORIN IN LITHOAUTOTROPHICALLY AND ORGANOHETEROTROPHICALLY GROWN RHODOBACTER-CAPSULATUS CELLS

Authors
WALDMANN U ROBLEDANO M WECKESSER J SCHULZ G
Citation
U. Waldmann et al., STRUCTURALLY IDENTICAL PORIN IN LITHOAUTOTROPHICALLY AND ORGANOHETEROTROPHICALLY GROWN RHODOBACTER-CAPSULATUS CELLS, FEMS microbiology letters, 131(3), 1995, pp. 343-346
Citations number
15
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
131
Issue
3
Year of publication
1995
Pages
343 - 346
Database
ISI
SICI code
0378-1097(1995)131:3<343:SIPILA>2.0.ZU;2-C
Abstract
The porin from lithoautotrophically grown Rhodobacter capsulatus 37b4 cells migrated identically to porin from organoheterotrophic cells on SDS-PAGE. Moreover, it behaved comparably in isoelectric focusing, and it was also EDTA-sensitive. Furthermore, the porins of the two growth conditions were essentially identical in amino acid composition and N -terminal amino acid sequence. A final proof for structural identity c ould be obtained by crystallization and structural analysis showing id entity in all non-hydrogen atoms.