AUTOMATED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF A NEURAMINIDASE INHIBITOR (GG167) IN HUMAN SERUM BY PRECOLUMN FLUORESCENCE DERIVATIZATION USING BENZOIN

A pre-column fluorescence derivatisation high-performance liquid chrom atographic method for the analysis of a neuraminidase inhibitor, GG167 , in human serum is described. GG167 was extracted from serum samples using Bond Elut SCX solid-phase extraction cartridges, followed by der ivatisation with benzoin prior to reversed-phase chromatography with f luorescence detection. This method has been automated using a Zymark r obot and used in the analysis of human serum samples from clinical stu dies. The method has been shown to be valid over a concentration range of 10-800 ng/ml using a 1-ml sample volume.