HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF '-DIHYDROXYBENZOPHENONE-2,4-DINITROPHENYLHYDRAZONE IN PLASMA

An analytical method has been developed for the determination of '-dih ydroxybenzophenone-2,4-dinitrophenylhydrazone (I, trade name A-007) in plasma. Plasma samples are primed with the internal standard, '-dihyd roxybenzophenone-2,4-dinitrophenylhydrazone (II), deproteinized with a cetonitrile, centrifuged and filtered prior to assay. The components a re then separated on a reversed-phase column with retention times of 4 .4 and 6.0 min for I and II, respectively. Ultraviolet detection at 36 5 nm was employed and little interference with the analyte or the inte rnal standard was noted from other plasma components. This method has been applied to the plasma of rats and monkeys dosed for pharmacokinet ic and toxicity studies.