AUTOMATED DNA PURIFICATION AND AMPLIFICATION FROM BLOOD-STAINED CARDSUSING A ROBOTIC WORKSTATION

Authors
BELGRADER P DELRIO SA TURNER KA MARINO MA WEAVER KR WILLIAMS PE
Citation
P. Belgrader et al., AUTOMATED DNA PURIFICATION AND AMPLIFICATION FROM BLOOD-STAINED CARDSUSING A ROBOTIC WORKSTATION, BioTechniques, 19(3), 1995, pp. 426
Citations number
12
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
19
Issue
3
Year of publication
1995
Database
ISI
SICI code
0736-6205(1995)19:3<426:ADPAAF>2.0.ZU;2-F
Abstract
A new method for the purification of DNA on blood-stained cards was de veloped. This method was implemented into a high-throughput automated system using a Biomek(TM) 1000 robotic workstation. In addition, the p rocesses of DNA purification and amplification were coupled into a com pletely automated and uninterrupted prototype system and the resultant PCR products generated by this system were subjected to automated des alting for capillary electrophoresis analysis.