DETERMINATION OF ARGININOSUCCINATE LYASE IN HUMAN SERUM BY ION-PAIR REVERSED-PHASE LIQUID-CHROMATOGRAPHY

Authors
CANEPARI S CARUNCHIO V GIRELLI AM MESSINA A
Citation
S. Canepari et al., DETERMINATION OF ARGININOSUCCINATE LYASE IN HUMAN SERUM BY ION-PAIR REVERSED-PHASE LIQUID-CHROMATOGRAPHY, BMC. Biomedical chromatography, 9(4), 1995, pp. 171-174
Citations number
12
Categorie Soggetti
Chemistry Analytical","Pharmacology & Pharmacy",Biology,"Biochemical Research Methods
Journal title
BMC. Biomedical chromatography → ACNP
ISSN journal
02693879
Volume
9
Issue
4
Year of publication
1995
Pages
171 - 174
Database
ISI
SICI code
0269-3879(1995)9:4<171:DOALIH>2.0.ZU;2-3
Abstract
A new assay for argininosuccinate lyase based on the separation of the enzymatic reaction mixture components by an ion-pair reversed phase m echanism is reported. The determination of enzyme activity is performe d after direct injection by UV detection of the fumarate formed, The c hromatographic analysis time is 8 min and a detection limit of 0.4 U/L is achieved. The HPLC method is highly accurate, sensitive and precis e. The simple procedure makes this method suitable for the routine det ermination of ASAL activity in human serum samples.