Hog cholera virus (HoCV) 5' terminus of the ALD and GPE(-) strains wer
e analyzed by using rapid amplification of cDNA end method (5'RACE). A
n additional nine nucleotides were found at the 5' termini of genomic
RNA in the ALD and GPE(-) strains of HoCV. These nine nucleotides were
also conserved in BVDV and were suggested to form a hairpin structure
at the 5' terminus by computer-assisted analysis. It seems possible t
hat the secondary structure and/or the 5' terminus sequence has a sign
ificant role in the HoCV virus genome.