AN ELISA FOR GRANCALCIN, A NOVEL CYTOSOLIC CALCIUM-BINDING PROTEIN PRESENT IN LEUKOCYTES

Grancalcin is a newly discovered cytosolic calcium-binding protein, be longing to the group of EF-hand proteins. Grancalcin is specifically a ssociated with cells originating in the bone marrow. Grancalcin binds reversibly to secretory vesicles and plasma membranes in human neutrop hils and might therefore play a role in the regulation of vesicle/gran ule exocytosis. We describe here the production of recombinant grancal cin, the generation of antibodies to the protein and the development o f a specific, accurate and sensitive ELISA for the detection of human leukocytic grancalcin. This ELISA may be useful for monitoring leukocy te infiltration into tissues.