EXPERIMENTAL STUDIES OF SOME MICRORHEOLOGICAL FACTORS IN SERIOUS BRAIN ISCHEMIA AFTER STOBADINE PRETREATMENT

Serious brain ischemia was induced by occlusion of cerebral arteries i n dogs. The occlusion time was 7 min. The blood was collected at vario us intervals of reperfusion (5, 60, 180, 240 min and 24 h). Thirty min utes before ischemization, stobadine was given (1, 2, or 5 mg/kg). The changes of erythrocyte membrane fluidity were evaluated using colloid -osmotic hemolysis induced by brilliant cresyl blue. In the control gr oup (without stobadine) the colloid-osmotic hemolysis was significantl y increased immediately after ischemization and after 5 and 60 min. Ho wever, after 240 min of reperfusion, a significant decrease of hemolys is was observed. The increase of colloid-osmotic hemolysis after ische mization in the control group was prevented after stobadine pretreatme nt. The thrombotization of microcirculation that was observed in the c ontrol group was not present after stobadine pretreatment.