CYTOGENETIC ANALYSIS OF UNCLEAVED HUMAN O OCYTES AFTER SPERMATOZOA MICROINJECTION

After standard in vitro fertilization (IVF) chromosome analysis of unc leaved oocytes has shown that in about 80 % of the cases, there was no fertilization at all while in 10 % of the cases there was a premature chromosome condensation and a development arrest. After micro-injecti on our results suggest that both the technique itself and the patholog ies which require micro-injection can influence the results, On the ot her hand about 20-30 % of the metaphase II oocytes are cytogenetically abnormal after IVF attempt while ICSI might increase chromosome break age. Our results suggest that both the technique used and the gamete p athologies can influence the different steps of development arrest.