ROOM-TEMPERATURE PHOSPHORESCENCE PROPERTIES OF DANSYL CHLORIDE AND ITS DERIVATIVES

Emission conditions and spectral properties of 5-dimethylaminonaphthal ene sulfonylchloride (DNS-Cl) for micelle stabilized room temperature phosphorescence (MS-RTP) and solid substrate room temperature phosphor escence (SS-RTP) are studied. In sodium dodecyl sulphate micelle syste ms, MS-RTP emission of DNS-Cl can be induced with TINO3 as heavy atom perturber and Na2SO3 for deoxygenation, lambda(ex)/lambda(em) = 328/55 8 nm. The intensity of RTP is linear with the concentration of DNS-Cl in the range of 1 X 10(-7) to 4 X 10(-5) mol l(-1). On several solid s ubstrates, the SS-RTP of DNS-Cl can be achieved with lead acetate (Pb( AC)(2)) as heavy atom perturber. On filter paper and for lambda(ex)/la mbda(em) = 335/575 nm, the linear range is 12 to 300 pmol DNS-Cl in 0. 3 mu l sample volume and the limit of detection is 12 pmol. On the oth er hand, the MS-RTP emission of derivative products for amino acid and ethanolamine can also be induced. Compared with that of DNS-Cl, not o nly the lambda(ex)/lambda(em) of the derivative products show a clear shift to longer wavelengths, but also the optimal pH increased from 7 to 10. These results show that DNS-Cl can be used not only as a fluore scence reagent, but also for RTP.