ELONGATION-FACTOR 1-ALPHA CONCENTRATION IS HIGHLY CORRELATED WITH THELYSINE CONTENT OF MAIZE ENDOSPERM

Lysine is the most limiting essential amino acid in cereals, and for m any years plant breeders have attempted to increase its concentration to improve the nutritional quality of these grains, The opaque2 mutati on in maize doubles the lysine content in the endosperm, but the mecha nism by which this occurs is unknown, We show that elongation factor 1 alpha(EF-1 alpha) is overexpressed in opaque2 endosperm compared with its normal counterpart and that there is a highly significant correla tion between EF-1 alpha concentration and the total lysine content of the endosperm. This relationship is also true for two other cereals, s orghum and barley, It appears that genetic selection for genotypes wit h a high concentration of EF-1 alpha can significantly improve the nut ritional quality of maize and other cereals.