Ee. Nelson et al., EFFECTS OF TRICHODERMA SPP AND AMMONIUM SULFAMATE ON ESTABLISHMENT OFARMILLARIA-LUTEOBUBALINA ON STUMPS OF EUCALYPTUS-DIVERSICOLOR, Mycological research, 99, 1995, pp. 957-962
Stumps of karri (Eucalyptus diversicolor) were simultaneously inoculat
ed with the pathogen Armillaria luteobubalina and five combined isolat
es of Trichoderma spp, to examine their potential for biological contr
ol. Trichoderma inoculum was placed in holes drilled into the stumps o
r in slashes cut in the bark and sapwood about 5 cm above the ground l
ine, either as (a) conidia suspended in an agar matrix, or (b) mycelia
and conidia encased in calcium alginate beads and suspended in an aga
r matrix. Half the stumps were poisoned with application of ammonium s
ulphamate (AMS) to the freshly cut stump top. The stumps were harveste
d 2 yr after inoculations. Observations and isolations made from disse
cted root and stem sections indicated that (a) AMS treatment significa
ntly increased colonization of stump roots by A. luteobubalina, and (b
) Trichoderma spore inoculum, v. bead inoculum, placed in drill holes
in the stumps resulted in a significant reduction in root colonization
by A. luteobubalina. Spore inoculum may have adversely affected fruit
ing of A. luteobubalina on stumps as well, since none of the 20 stumps
on which A. luteobubalina fruited was inoculated with spores.