ORGAN FORMATION AND PLANT-REGENERATION FR OM VARIOUS TISSUES OF TARA VINE (ACTINIDIA-ARGUTA PLANCH) CULTURED IN-VITRO

Explants excised from an internode, immature and mature leaves, petiol e, immature seed and fruit, and an in vitro-differentiated root of tar a vine (Actinidia arguta Planch.) were cultured on Miller's medium con taining sucrose (30 g . liter(-1)) and gelrite (2 g . liter(-1)), and 15 combinations of BA (0, 1, 10 mu M) and NAA (0, 0.1, 1, 10, 100 mu M ). Callus formed on all explants except that from a mature leaf. Adven titious buds formed on the surface layer of the calli which were induc ed from the cortex of an internodal segment. After 60 days of culture, shoots emerged from the adventitious buds. Shoot formation was suppre ssed with an increase in NAA concentration, but this suppression tende d to be reversed by the addition of BA. When transferred to the medium supplemented with combinations of 0, 2, 4, 6, 8 and 10 mu M NAA and 1 and 10 mu M BA, the in vitro-differentiated shoots formed roots in a medium with 4 mu M NAA and 1 mu M BA, and regenerated into plantlets a fter 45 days of subculture. For acclimation, the plantlets were transp lanted to a synthetic soil (soil : vermiculite, 4 : 1, v/v); nearly al l survived, becoming lianoid 40 days later.