FAILURE OF POSTSYNAPTIC SPECIALIZATION TO DEVELOP AT NEUROMUSCULAR-JUNCTIONS OF RAPSYN-DEFICIENT MICE

OF numerous synaptic components that have been identified, perhaps the best-studied are the nicotinic acetylcholine receptors (AChRs) of the vertebrate neuromuscular junction(1). AChRs are diffusely distributed on embryonic myotubes, but become highly concentrated (similar to 10, 000 mu m(-2)) in the postsynaptic membrane as development proceeds. At least two distinct processes contribute to this accumulation. One is local synthesis: subsynaptic muscle nuclei transcribe AChR subunit gen es at higher rates than extrasynaptic nuclei, so AChR messenger RNA is concentrated near synaptic sites(2,3). Second, once AChRs have been i nserted in the membrane, they form high-density clusters by tethering to a subsynaptic cytoskeletal complex. A key component of this complex is rapsyn, a peripheral membrane protein of relative molecular mass 4 3K (refs 4, 5), which is precisely colocalized with AChRs at synaptic sites from the earliest stages of neuromuscular synaptogenesis(6). In heterologous systems, expression of recombinant rapsyn leads to cluste ring of diffusely distributed AChRs, suggesting that rapsyn may contro l formation of clusters(7,8). To assess the role of rapsyn in vivo, we generated and characterized mutant mice with a targeted disruption of the Rapsn gene. We report that rapsyn is essential for the formation of AChR clusters, but that synapse-specific transcription of AChR subu nit genes can proceed in its absence.