Displacement of lactate dehydrogenase from dye-affinity matrices with
poly(ethyleneimine) (PEI) was shown to be an effective elution strateg
y. It resulted in better recoveries and sharper elution profiles than
traditional non-specific elution while the purification factors were u
nchanged. The elution is assumed to proceed via displacement of bound
protein by PEI when the polymer binds to the dye-ligands. Complete elu
tion of bound protein is a characteristic feature of such a mechanism.
Hence displacement with PEI may be a promising strategy for eluting p
roteins with reported low recoveries in dye-affinity chromatography pr
otocols.