ENANTIOMERIC RESOLUTION OF DERIVATIZED DL-AMINO ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING A BETA-CYCLODEXTRIN CHIRAL STATIONARY-PHASE - A COMPARISON BETWEEN DERIVATIZATION LABELS

A chiral stationary phase with immobilized beta-cyclodextrin was used for the liquid chromatographic separation of amino acid derivatives in to their enantiomers. Several reagents differing in size, structure an d linking group (i.e. the isothiocyanates PITC, NITC, DNITC and DABITC , the activated ester AQC, and the sulfonyl chlorides DNS-Cl and DABS- Cl) were used to derivatize the amino groups of alpha-amino acids prio r to separation. A compilation of the selectivity data obtained with t he different labels is given as guideline in selecting appropriate lab els for a particular separation problem.