ENANTIOMERIC SEPARATION OF AMPHETAMINE RELATED DRUGS BY CAPILLARY ZONE ELECTROPHORESIS USING NATIVE AND DERIVATIZED BETA-CYCLODEXTRIN AS CHIRAL ADDITIVES

Amphetamine, methamphetamine and several ring-substituted analogs whic h are under governmental regulations have been separated by capillary zone electrophoresis employing native and various substituted beta-cyc lodextrins as additives to the background electrolyte. The following c hiral selectors were used: native beta-cyclodextrin, heptakis-(2,6-di- O-methyl)-beta-cyclodextrin, heptakis-(2,3,6-tri-O-methyl)-beta-cyclod extrin, (2-hydroxy)propyl-beta-cyclodextrin and carboxymethyl-beta-cyc lodextrin. The amphetamines were separated without derivatization. Sep arations are reported with respect to the kind of chiral selector. Nat ive beta-cyclodextrin and carboxymethyl-beta-cyclodextrin turned out t o give optimal resolutions within only a few minutes. This direct meth od is compared with the indirect method separating the diastereomeric Marfey's derivatized amphetamines by means of non-chiral sodium dodecy lsulfate micelles.