REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THEQUANTITATIVE-DETERMINATION OF ALKYLBIS(2-BENZOTHIAZOLYLSULFEN)AMIDES

An isocratic, reversed-phase HPLC procedure was developed for the simu ltaneous determination of isopropyl-, tert.-butyl-, tert.-amyl-, cyclo hexylbis(2-benzothiazolylsulfen) amides. Quantitation is performed on a C-18 bonded-phase column (Separon SGX C-18, 5 mu m) using N-dicycloh exyl-2-benzothiazolesulfenamide as internal standard followed by UV ph otodiode-array detection. The precision (n=7) for all derivatives of a lkylbis(2-benzothiazolylsulfen)amides is within 1.5%. Identification o f the compounds also in the mixtures was done by NMR spectroscopy.