MEASUREMENT OF DESMOSINE AND ISODESMOSINE BY CAPILLARY ZONE ELECTROPHORESIS

Desmosine (DES) and isodesmosine (IDE) were separated and quantitated by a simple and sensitive capillary zone electrophoretic (CZE) method, using hydrostatic injection and direct UV detection at 254 or 185 nm. Two different electrophoretic mobilities for the two isoforms were ob served in 90 mM phosphoric acid pH 2.2. The presence of a mixture of a mino acids in the sample did not affect the separation of DES and IDE. The method was successfully used to quantitate the amounts of DES and IDE in elastin hydrolysates.