EFFECT OF DIETARY FLAXSEED ON FATTY-ACID COMPOSITION, SUPEROXIDE, NITRIC-OXIDE GENERATION AND ANTILISTERIAL, ACTIVITY OF PERITONEAL, MACROPHAGES FROM FEMALE SPRAGUE-DAWLEY RATS
Us. Babu et al., EFFECT OF DIETARY FLAXSEED ON FATTY-ACID COMPOSITION, SUPEROXIDE, NITRIC-OXIDE GENERATION AND ANTILISTERIAL, ACTIVITY OF PERITONEAL, MACROPHAGES FROM FEMALE SPRAGUE-DAWLEY RATS, Life sciences, 60(8), 1997, pp. 545-554
Citations number
39
Categorie Soggetti
Biology,"Medicine, Research & Experimental","Pharmacology & Pharmacy
The impact of ground flaxseed (FS) or flaxseed meal (FSM) diets on the
fatty acid composition and functions of rat peritoneal exudate cells
(PEG) was determined. Female weanling Sprague-Dawley rats (10/group) w
ere fed isocaloric AIN-76 diets supplemented with 0.0, 10.0% (w/w) FS
or 6.2% (w/w) FSM. A the end of 56-days, rat serum and thiogrycollate-
elicited PEC were analyzed for total lipid fatty acids. Production of
nitric oxide (NO) and superoxide (O-2(-)), Listeria monocytonenes (LM)
phagocytic index and antilisterial activity of resident PEC were also
assessed. A significant increase in a-linolenic (C18:3), eicosapentan
oic (C20:5) and docosahexanoic (C22:6) acids, as well as a significant
reduction in arachidonic acid (C20:4) was observed in the serum of ra
ts fed 10% FS. Dietary FS caused a significant reduction in palmitic a
cid (C16:0) and an increase in stearic acid (C18:0) of PEC. Defatted F
SM produced a significant increase in long chain fatty acids, which in
cluded eicosadienoic acid (C20:2) in PEC and C22:6 in serum. PEC from
rats fed 10.0% FS produced significantly less (about 50%) O-2(-) in re
sponse to phorbol myristate acetate (PMA), than did PEC from control a
nimals; dietary treatment had no effect on O-2(-) in response to LM. F
SM had no impact on the O-2(-) production by PEC in response to PMA or
LM. Antilisterial activity of PEC was determined by comparing bacteri
al uptake after 1 hr with recovery 24 hrs later. Despite comparably eq
uivalent bacterial uptake, few viable intracellular LM were recovered
at T=24 for all test samples, indicating that, regardless of the dieta
ry treatment, PEC were able to handle the in vitro LM infection. This
bacterial clearance was accompanied by equivalent NO generation by PEC
from each dietary group in response to LM. Summarily, dietary FS prod
uced significant changes in fatty acid composition of serum and PEG, i
nhibited O-2(-) generation by PEG, and was ineffectual to both NO prod
uction by and antilisterial activity of PEC.