DETERMINATION BY NMR-SPECTROSCOPY OF THE STRUCTURE AND CONFORMATIONALFEATURES OF THE ENTEROBACTERIAL COMMON ANTIGEN ISOLATED FROM ESCHERICHIA-COLI

Authors
BRUIX M JIMENEZBARBERO J CRONET P
Citation
M. Bruix et al., DETERMINATION BY NMR-SPECTROSCOPY OF THE STRUCTURE AND CONFORMATIONALFEATURES OF THE ENTEROBACTERIAL COMMON ANTIGEN ISOLATED FROM ESCHERICHIA-COLI, Carbohydrate research, 273(2), 1995, pp. 157-170
Citations number
32
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Carbohydrate research → ACNP
ISSN journal
00086215
Volume
273
Issue
2
Year of publication
1995
Pages
157 - 170
Database
ISI
SICI code
0008-6215(1995)273:2<157:DBNOTS>2.0.ZU;2-F
Abstract
Complete H-1 and C-13 spectrum of a polysaccharide isolated from Esche richia coli, which is the major component of the enterobacterial commo n antigen, has been analyzed through two-dimensional nuclear magnetic resonance spectroscopy. In addition, distance constraints from NOESY a nd ROESY experiments have been combined with molecular dynamic simulat ions to determine its major conformation in water solution. Data resul ting from both free dynamic simulations and restrained dynamic simulat ions have been compared with experimental data and discussed.