M. Garzon et al., DETERMINATION OF PENTAMIDINE IN SERUM AND URINE BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY, Journal of chromatography B. Biomedical sciences and applications, 688(1), 1997, pp. 135-142
Citations number
33
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A number of parameters influencing the electrokinetic processing of pe
ntamidine by micellar electrokinetic chromatography (MEKC) were studie
d in order to develop an analytical method for this compound. The para
meters considered were: pH, ionic strength, and SDS concentration of e
lectrolyte, temperature and working voltage. On the basis of the resul
ts obtained, the best analytical conditions for the detection of penta
midine in serum and urine by MEKC were determined. Analysis by MEKC pe
rmitted determination of the drug in 10 min. Good linearity, reproduci
bility and accuracy were obtained in the range 0-30 mu g/ml for both s
amples, with a correlation coefficient r greater than or equal to 0.99
98 and a recovery of 87-92% in serum and 90-108.9% in urine. We examin
ed the metabolism of pentamidine using rat liver homogenates in order
to exclude any possible interference of metabolites in the analysis of
pentamidine.