HIGH-RESOLUTION MAPPING OF 10 UNIQUE DNA-SEQUENCES TO HUMAN-CHROMOSOME-3 SUBREGIONS BY IN-SITU HYBRIDIZATION

Tell single-copy DNA probes derived from a human chromosome 3-specific genomic library were mapped by in situ hybridization to subregions of this chromosome. Seven sequences were assigned to subregions of 3q an d two sequences were assigned to subregions of 3p. One single-copy DNA probe was assigned to the centromeric region of chromosome 3 by South ern blot analysis of DNA isolated from a somatic cell hybrid containin g centromeric sequences of this chromosome. These DNA clones mapped by in situ hybridization can provide useful landmarks for mapping variou s disease loci on chromosome 3. They may also be useful for the genera tion of physical and genetic maps.