Sj. Marsh et al., SYNERGISTIC REGULATION OF A NEURONAL CHLORIDE CURRENT BY INTRACELLULAR CALCIUM AND MUSCARINIC RECEPTOR ACTIVATION - A ROLE FOR PROTEIN-KINASE-C, Neuron, 15(3), 1995, pp. 729-737
Using perforated patch recordings in combination with intracellular Ca
2+ ([Ca2+](i)) fluorescence measurements, we have identified a delayed
Ca2+-dependent Cl- current in a mammalian sympathetic ganglion cell.
This Cl- current is induced by the synergistic action of Ca2+ and diac
ylglycerol (DAG) and is blocked by inhibitors of protein kinase C. As
a result, the current can be induced by acetylcholine through the conj
oint activation of nicotinic receptors (to produce a rise in [Ca2+](i)
) and muscarinic receptors (to generate DAG). This demonstrates an unu
sual form of synergism between the two effects of a single transmitter
mediated via separate receptors operating within a time scale that co
uld be of physiological significance.