FURTHER STUDY ON THE USE OF UNCHARGED BETA-CYCLODEXTRIN POLYMER IN CAPILLARY ELECTROPHORESIS - ENANTIOMERIC SEPARATION OF SOME ALPHA-HYDROXY ACIDS

Uncharged beta-cyclodextrin polymer was used as chiral selector for th e enantiomeric separation of same alpha-hydroxy acids by capillary ele ctrophoresis. Complexation and enantiomeric resolution of mandelic aci d, m-hydroxy and p-hydroxymandelic acid, 3,4-dihydroxymandelic acid, a s well as 2- and 3-phenyllactic acid were studied, changing the concen tration of the beta-cyclodextrin polymer added to the background elect rolyte at different pH in the range of 4.5-7. Furthermore, the effects of the concentration of the background electrolyte, column temperatur e, and applied voltage on chiral resolution were also examined. The be st enantiomeric separations were obtained using a background electroly te at pH 6 containing 100 mg/mL of beta-cyclodextrin polymer.