L. Benbacer et al., INTERSPECIES AMINOPEPTIDASE-N CHIMERAS REVEAL SPECIES-SPECIFIC RECEPTOR RECOGNITION BY CANINE CORONAVIRUS, FELINE INFECTIOUS PERITONITIS VIRUS, AND TRANSMISSIBLE GASTROENTERITIS VIRUS, Journal of virology, 71(1), 1997, pp. 734-737
Ne report that cells refrectory to canine coronavirus (CCV) and feline
infectious peritonitis virus (FIPV) became susceptible when transfect
ed with a chimeric aminopeptidase-N (APN) cDNA containing a canine dom
ain between residues 643 and 841. This finding shows that APN recognit
ion by these viruses is species related and associated with this C-ter
minal domain. The human/canine APN chimera was also able to confer sus
ceptibility to the porcine transmissible gastroenteritis virus (TGEV),
whereas its human/porcine homolog failed to confer susceptibility to
CCV and FIPV. A good correlation was observed between the capacity of
CCV, FIPV, and TGEV to recognize the different interspecies APN chimer
as and their ability to infect cells derived from the relevant species
. As an exception, TGEV was found to use a human/bovine APN chimera as
a receptor although itself unable to replicate in bovine cells.