FUSION OF BOVINE LEUKEMIA-VIRUS WITH TARGET-CELLS MONITORED BY R18 FLUORESCENCE AND PCR ASSAYS

Authors
ZARKIK S DEFRISEQUERTAIN F PORTETELLE D BURNY A RUYSSCHAERT JM
Citation
S. Zarkik et al., FUSION OF BOVINE LEUKEMIA-VIRUS WITH TARGET-CELLS MONITORED BY R18 FLUORESCENCE AND PCR ASSAYS, Journal of virology, 71(1), 1997, pp. 738-740
Citations number
28
Categorie Soggetti
Virology
Journal title
Journal of virology → ACNP
ISSN journal
0022538X
Volume
71
Issue
1
Year of publication
1997
Pages
738 - 740
Database
ISI
SICI code
0022-538X(1997)71:1<738:FOBLWT>2.0.ZU;2-4
Abstract
PCR and R18 fluorescence dequenching assays have been combined to moni tor the kinetics of fusion of bovine leukemia virus with target cells (CC81, OVK, or Raji). Antibodies raised against gp51 allow us to demon strate that not only the hydrophobic N-terminal domain of the transmem brane glycoprotein gp30 but also specific domains of gp51 (amino acids 39 to 103) are involved in bovine leukemia virus-cell fusion.