J. Corsini et al., EXPRESSION OF PARVOVIRUS LUIII NS1 FROM A SINDBIS REPLICON FOR PRODUCTION OF LUIII-LUCIFERASE TRANSDUCING VIRUS, Virus research, 46(1-2), 1996, pp. 95-104
In order to develop an alternative packaging system for recombinant pa
rvoviruses, the gene for the major nonstructural protein (NS1) of parv
ovirus LuIII was inserted into a Sindbis replicon vector. Cells infect
ed with recombinant SinNS1 virus produced NS1 RNA from the Sindbis 26S
promoter and expressed NS1 protein which was able to transactivate a
parvovirus P38 promoter. Co-transfections of Sindbis-NS1 RNA together
with a packageable LuIII transducing genome and a coat protein express
ion plasmid generated detectable levels of LuIII-luciferase transducin
g virus. These levels could be increased by using a capsid expression
plasmid that was also capable of expressing NS2. These results show th
at a multi-functional parvovirus protein expressed from a Sindbis RNA
molecule can be used to produce recombinant parvoviruses. Copyright (C
) 1996 Elsevier Science B.V.