MOUSE CELLS EXPRESSING HUMAN INTERCELLULAR-ADHESION MOLECULE-1 ARE SUSCEPTIBLE TO INFECTION BY COXSACKIEVIRUS A21

Competitive viral binding assays have revealed previously that coxsack ievirus A21 (CAV21) and human rhinovirus 14 (HRV14) share a common cel l surface receptor. More recently, intercellular adhesion molecule-1 ( ICAM-1) has been identified as the cellular receptor for HRV-14. Also, anti-ICAM-1 monoclonal antibodies (MAbs) blocked infection by HRV14, CAV13, CAV18, and CAV21, suggesting that these viruses share this rece ptor; however, this has never been established by more direct methods. In this study we show conclusively that CAV21 binds to ICAM-1 and tha t MAbs directed against the N-terminal domain of the molecule inhibit this attachment. Furthermore, we show that the specific interaction be tween ICAM-1 and 160S CAV21 virions induces formation of 135S A partic les. Finally, we show transfection of normally nonsusceptible mouse L cells with human ICAM-1 cDNA renders them susceptible to infection by CAV21.