EFFICIENT MULTIPLICATION OF A SEMLIKI-FOREST-VIRUS CHIMERA CONTAININGSINDBIS VIRUS SPIKES

Authors
SMYTH J SUOMALAINEN M GAROFF H
Citation
J. Smyth et al., EFFICIENT MULTIPLICATION OF A SEMLIKI-FOREST-VIRUS CHIMERA CONTAININGSINDBIS VIRUS SPIKES, Journal of virology, 71(1), 1997, pp. 818-823
Citations number
28
Categorie Soggetti
Virology
Journal title
Journal of virology → ACNP
ISSN journal
0022538X
Volume
71
Issue
1
Year of publication
1997
Pages
818 - 823
Database
ISI
SICI code
0022-538X(1997)71:1<818:EMOASC>2.0.ZU;2-H
Abstract
Using the Semliki Forest virus (SFV) and Sindbis virus (SIN) cDNAs we have constructed recombinants in which the spike genes were exchanged. Analyses of expression showed that the SFV/SIN(spike) RNA directed ef ficient assembly of infectious virus, whereas the reciprocal SIN/SFV(s pike) RNA was completely unable to assemble virus. This was apparently due to a defective capsid-spike interaction.