Hjj. Mengelers et al., COGNATE INTERACTION BETWEEN HUMAN-LYMPHOCYTES AND EOSINOPHILS IS MEDIATED BY BETA-2-INTEGRINS AND VERY LATE ANTIGEN-4, The Journal of laboratory and clinical medicine, 126(3), 1995, pp. 261-268
Citations number
43
Categorie Soggetti
Medical Laboratory Technology","Medicine, General & Internal
Here the cognate interactions between human eosinophils and lymphocyte
s are studied. These interactions were measured in a double-colored FA
CS analysis by applying fluorescent red eosinophils (stained with hydr
oethidine, 40 mu mol/L) and fluorescent green lymphocytes (stained wit
h sulfidofluorescein diacetate, 100 mu mol/L) in a ratio of 1:3, When
normal eosinophils were mixed with a total lymphocyte preparation in s
tirred suspensions (37 degrees C), no physical interaction was present
between both cell types. However, the addition of phytohemagglutinin
and PMA resulted in a clear aggregation response between both cell typ
es (up to 30% of the eosinophils interacted with lymphocytes after 15
minutes), CD8(+)- and CD4(+)-positive T cells contributed equally to t
he heterotypic aggregation response. It is interesting that when lymph
ocytes were pretreated with phytohemagglutinin or eosinophils with pho
rbol myristate acetate and subsequently washed, the cells still intera
cted with unstimulated counterparts. The heterotypic interaction betwe
en lymphocytes and eosinophils is blocked by monoclonal antibodies dir
ected against the beta-chain of the beta(2)-integrins (CLB LFA1/1; CD1
8) and the alpha-chain of very late antigen-4 (VLA-4) (HP2/1; CD49d),
indicating that both the beta(2)-integrins and VLA-4 contributed to th
is heterotypic interaction. When eosinophils bound to PHA-treated lymp
hocytes, the cells exhibited an activated phenotype that was character
ized by an enhanced expression of CD66b and CD11b, The cells interacte
d with each other provided that an intact cellular metabolism was pres
ent-that is, no interaction was seen after treatment with the glycolys
is inhibitor sodium mono-iodoacetate, In conclusion, our findings indi
cate that eosinophils can physically interact with lymphocytes provide
d that the beta(2)-integrins and/or VLA-4 are in an activated state.