ACTIVATION OF PROTEIN-KINASE-C AND THE INVOLVEMENT OF PROSTAGLANDIN E(2) IN THE INHIBITION OF OSTEOSARCOMA-DERIVED CELL ALKALINE-PHOSPHATASE ACTIVITY

Authors
FUKUDA K UENO M SAITOH M NISHIOKA S TANAKA S
Citation
K. Fukuda et al., ACTIVATION OF PROTEIN-KINASE-C AND THE INVOLVEMENT OF PROSTAGLANDIN E(2) IN THE INHIBITION OF OSTEOSARCOMA-DERIVED CELL ALKALINE-PHOSPHATASE ACTIVITY, The Journal of laboratory and clinical medicine, 126(3), 1995, pp. 269-274
Citations number
33
Categorie Soggetti
Medical Laboratory Technology","Medicine, General & Internal
Journal title
The Journal of laboratory and clinical medicine → ACNP
ISSN journal
00222143
Volume
126
Issue
3
Year of publication
1995
Pages
269 - 274
Database
ISI
SICI code
0022-2143(1995)126:3<269:AOPATI>2.0.ZU;2-S
Abstract
We present evidence for the presence of specific, high-affinity bindin g sites for tritiated phorbol 12,13-dibutyrate on osteosarcoma-derived (HT-3) cells. Activation of protein kinase C by a phorbol ester resul ted in an inhibition of alkaline phosphatase activity and the accumula tion of prostaglandin E(2). Indomethacin blocked prostaglandin E(2) pr oduction and enhanced alkaline phosphatase activity, These data sugges t that prostaglandin E(2) is enhanced by activation of protein kinase C, and in turn, alkaline phosphatase activity is reduced.