LABELING PROTEINS AT HIGH SPECIFIC ACTIVITY USING N-SUCCINIMIDYL 4-[F-18](FLUOROMETHYL) BENZOATE

Authors
LANG L ECKELMAN WC
Citation
L. Lang et Wc. Eckelman, LABELING PROTEINS AT HIGH SPECIFIC ACTIVITY USING N-SUCCINIMIDYL 4-[F-18](FLUOROMETHYL) BENZOATE, Applied radiation and isotopes, 48(2), 1997, pp. 169-173
Citations number
16
Categorie Soggetti
Nuclear Sciences & Tecnology","Radiology,Nuclear Medicine & Medical Imaging","Chemistry Inorganic & Nuclear
Journal title
Applied radiation and isotopes
ISSN journal
09698043 → ACNP
Volume
48
Issue
2
Year of publication
1997
Pages
169 - 173
Database
ISI
SICI code
0969-8043(1997)48:2<169:LPAHSA>2.0.ZU;2-D
Abstract
High effective specific activity N-succinimidyl 4-[F-18](fluoromethyl) benzoate was prepared using a reversed phase HPLC procedure. Reversed phase HPLC removed several additional impurities not removed by normal phase HPLC, thereby increasing the effective specific activity. Small amount (<100 mu g) of sensitive proteins such as erythropoietin can b e labeled with this reagent in high yield without aggregation. Copyrig ht (C) 1997 Published by Elsevier Science Ltd