CULTURE MICROTITRATION - A SENSITIVE METHOD FOR QUANTIFYING LEISHMANIA-INFANTUM IN TISSUES OF INFECTED MICE

We developed a microtitration method to determine the parasite burdens in homogenized organs of mice infected with Leishmania infantum. This method proved more sensitive than direct enumeration of amastigotes i n stained organs, was appropriate for describing the kinetics of infec tion, and can be considered for physiopathological or pharmaceutical e xperimental studies.