N. Kurata et al., MUTATIONS IN THE V-MOS GENE ABOLISH ITS ABILITY TO INDUCE DIFFERENTIATION BUT NOT TRANSFORMATION, European journal of cell biology, 68(1), 1995, pp. 55-61
The v-mos oncogene product has the ability to induce differentiation i
n human monocytic leukemia U937 cells, thereby arresting cell prolifer
ation, and also exhibits transforming activity in mouse NIH3T3 cells.
Mutation in the v-mos gene consisting of one or two amino acid substit
utions in the putative ATP-binding domain impaired its differentiation
-inducing activity although mutant proteins showed rather higher level
s of autophosphorylation in vitro. Macrophage-specific characteristics
such as their morphology, expression of C3b receptor and Fc receptor,
and production of interleukin-1 beta and tumor necrosis factor alpha,
were equally diminished in cells transfected with mutant mos genes wh
en compared to those with intact v-mos. The ability of the gene to arr
est the proliferation of U937 cells was likewise diminished, while the
transforming efficiency of the intact and mutant mos genes mere essen
tially the same. These results suggest that the mos product functions
differently in cell differentiation and transformation.