A NATURAL GLYCOPROTEIN INHIBITOR (NIF) OF CD11B CD18 REDUCES LEUKOCYTE ADHESION IN THE LIVER AFTER HEMORRHAGIC-SHOCK/

This study was designed to assess the effect of neutrophil inhibitory factor (NIF), a novel specific inhibitor of CD11b/CD18 on hepatic leuk ocyte trafficking by intravital microscopy 5 h after hemorrhagic shock . Anesthetized rats were instrumented for invasive hemodynamical monit oring. Hemorrhagic shock was induced for 60 min by withdrawal of arter ial blood (mean arterial blood pressure = 40 mmHg). Rats were adequate ly resuscitated for 5 h to achieve a mean arterial blood pressure > 10 0 mmHg and were randomly assigned to blinded treatment with NIF or pla cebo control protein administered as a single intravenous bolus (10 mg /kg) at the time of resuscitation. Intrahepatic leukocyte adhesion was evaluated by in vivo fluorescence microscopy. There were no significa nt differences observed in hemodynamic parameters between the shock gr oups throughout the study, however, NIF significantly reduced firm leu kocyte adhesion in liver sinusoids. The results suggest that NIF may b e beneficial in the attenuation of the pathological shock-induced leuk ocyte adhesion.