Rl. Griffin et al., LECTIN FROM CODIUM FRAGILE SSP TOMENTOSOIDES CONJUGATED TO COLLOIDAL GOLD - A NEW HISTOCHEMICAL REAGENT, British journal of biomedical science, 52(3), 1995, pp. 225-227
A new histochemical reagent has been developed utilising a lectin from
a marine alga for the first time. Colloidal gold was coupled to the N
-acetyl-alpha-D-galactosamine specific lectin from the green alga Codi
um fragile ssp. tomentosoides. The lectin-gold conjugate bound to the
membranes of blood-group A(1) human erythrocytes which were used as a
model system. The bound complex could be detected, readily, by transmi
ssion electron microscopy. This novel reagent incorporating a lectin o
f low molecular weight (15 kDa) has potential value for studies of cel
l-surface topography of a variety of tissues.