LECTIN FROM CODIUM FRAGILE SSP TOMENTOSOIDES CONJUGATED TO COLLOIDAL GOLD - A NEW HISTOCHEMICAL REAGENT

Authors
GRIFFIN RL ROGERS DJ SPENCERPHILLIPS PTN SWAIN L
Citation
Rl. Griffin et al., LECTIN FROM CODIUM FRAGILE SSP TOMENTOSOIDES CONJUGATED TO COLLOIDAL GOLD - A NEW HISTOCHEMICAL REAGENT, British journal of biomedical science, 52(3), 1995, pp. 225-227
Citations number
15
Categorie Soggetti
Medical Laboratory Technology
Journal title
British journal of biomedical science → ACNP
ISSN journal
09674845
Volume
52
Issue
3
Year of publication
1995
Pages
225 - 227
Database
ISI
SICI code
0967-4845(1995)52:3<225:LFCFST>2.0.ZU;2-L
Abstract
A new histochemical reagent has been developed utilising a lectin from a marine alga for the first time. Colloidal gold was coupled to the N -acetyl-alpha-D-galactosamine specific lectin from the green alga Codi um fragile ssp. tomentosoides. The lectin-gold conjugate bound to the membranes of blood-group A(1) human erythrocytes which were used as a model system. The bound complex could be detected, readily, by transmi ssion electron microscopy. This novel reagent incorporating a lectin o f low molecular weight (15 kDa) has potential value for studies of cel l-surface topography of a variety of tissues.