Mp. Gomez et J. Segura, AXILLARY SHOOT PROLIFERATION IN CULTURES OF EXPLANTS FROM MATURE JUNIPERUS-OXYCEDRUS TREES, Tree physiology, 15(9), 1995, pp. 625-628
We developed procedures for the micropropagation of Juniperus oxycedru
s L. using shoot apices or nodal segments from mature plants. Of the m
edia and explants examined, best culture establishment was obtained wi
th shoot apices cultured on modified Schenk and Hildebrandt medium (SH
medium) without growth regulators; however, shoot multiplication was
only achieved when shoot apices isolated from shoots grown on SH mediu
m without growth regulators were subcultured on SH medium containing 0
.5 mu M benzyladenine. None of the auxins and methods tested for root
induction provided satisfactory results.