O. Cynshi et al., ACTION OF PHENOLIC ANTIOXIDANTS ON VARIOUS ACTIVE OXYGEN SPECIES, Journal of bioluminescence and chemiluminescence, 10(5), 1995, pp. 261-269
The action of phenolic antioxidants. such as probucol, on various acti
ve oxygen species was investigated using luminol chemiluminescence and
spin trapping with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The vario
us active oxygen species, including hydroxyl radicals(Fenton reaction)
, superoxide anions, singlet oxygen and hypochlorite ions were examine
d with phenolic antioxidants under aqueous and nonaqueous conditions.
Probucol showed a quenching effect on both superoxide anions and hypoc
hlorite ions in nonaqueous solution. However, it had no effect on hydr
oxyl radicals. a-Tocopherol, a natural phenolic antioxidant. showed a
stronger quenching effect on superoxide anions and hypochlorite ions t
han probucol, and quenched hydroxyl radicals in nonaqueous solution. F
urthermore, Trolox showed a quenching effect on all active oxygen spec
ies in both aqueous and nonaqueous solution. The antioxidants were stu
died under comparable conditions in a series of test systems and the r
eactivity profiles depicted as 'radar charts' which are helpful for ch
aracterizing antioxidant action.