A NEW TECHNIQUE FOR CONTINUOUS MEASUREMENT AND RECORDING OF GASTRIC POTENTIAL DIFFERENCE IN THE RAT - EVALUATION OF NSAID-INDUCED GASTRIC-MUCOSAL DAMAGE
C. Scarpignato et al., A NEW TECHNIQUE FOR CONTINUOUS MEASUREMENT AND RECORDING OF GASTRIC POTENTIAL DIFFERENCE IN THE RAT - EVALUATION OF NSAID-INDUCED GASTRIC-MUCOSAL DAMAGE, Journal of pharmacological and toxicological methods, 34(2), 1995, pp. 63-72
Disruption of the gastric mucosal barrier by the so-called ''barrier b
reakers'' such as ethanol, aspirin, and bile is associated with an inc
rease in gastric potential difference (GPD), that is, a decrease in it
s negativity. Because a good correlation between the degree of histolo
gical damage and changes in GPD has been observed, this parameter has
been used increasingly as an index of mucosal integrity. However, the
current methodology for measuring GPD is laborious due to the preparat
ion and checking of KCl-agarose bridges prior to each experiment, and
calculations-usually handmade-are time-consuming and inaccurate. In th
is paper, a new method allowing simultaneous measurement and recording
of GPD in the rat is described. The method allows a simultaneous reco
rding of intragastric pH and an automatic data analysis. The new techn
ique has been validated by studying mucosal damage induced by aspirin
and other nonsteroidal anti-inflammatory drugs (NSAIDs) (namely indome
thacin and droxicam) as well as the mucosal protective activity of an
antacid and sucralfate. The similarity between the results obtained in
this rat model and those derived from human experiments clearly show
that the developed methodology yields results that are predictive for
human pharmacology.