2ND-ORDER DERIVATIVE SPECTROPHOTOMETRIC ASSAY FOR IMIPRAMINE HYDROCHLORIDE AND DIAZEPAM IN PURE ADMIXTURES AND IN DOSAGE FORMS

The determination of imipramine HCl and diazepam in tablets by derivat ive spectrophotometry is described. The drugs in combined preparations have been quantified using the second-order derivative spectra of the ir solutions in 0.1 M HCl. The method has been applied to pure drug mi xtures as well as commerical preparations and was found to be precise and reproducible. Compliance of Beer's Law was observed in the concent ration range of 10-70 mu g ml(-1) for imipramine HCl and 2-8 mu g ml(- 1) for diazepam. Lower limits of detection at the 95% confidence level were 1.96 mu g ml(-1) for imipramine HCl and 0.21 mu g ml(-1) for dia zepam.