MICELLAR-ENHANCED SYNCHRONOUS-DERIVATIVE FLUORESCENCE DETERMINATION OF DERIVATIZED FOLIC-ACID IN PHARMACEUTICAL PREPARATIONS

A sensitive and inexpensive micellar-enhanced synchronous-derivative s pectrofluorimetric method is described for the determination of folic acid in pharmaceutical preparations. The method is based upon derivati zation of the vitamin with fluorescamine in acid solution and enhancem ent of the fluorescence with a surfactant. Linear fluorimetric analyti cal curves were obtained for folic acid concentrations of 0.05-6 mu g ml(-1) (detection limit 0.012 mu g ml(-1)); the RSD for 1 mu g ml(-1) of folic acid was 2.82%. The method has been applied to the determinat ion of folic acid synthetic mixtures and pharmaceutical preparations.