M. Fellner, INFLUENCE OF THE ANTIBIOTIC CIPROFLOXACIN ON CULTURE OF ALLIUM-LONGICUSPIS CALLUS-DERIVED PROTOPLASTS, Annals of botany, 76(3), 1995, pp. 219-223
A major problem of in vine plant culture techniques is chronic contami
nation by microorganisms. Calli derived from basal parts of leaves of
Allium longicuspis Regel (Alliaceae) and cultured in a medium without
antibiotic contain most probably latent contaminating microorganisms.
These calli were used as the source material for isolation and culture
of protoplasts. Isolated protoplasts were cultured in the presence of
the antibiotic ciprofloxacin, and the protoplast viability, cell wall
regeneration and cell division were studied as a function of the anti
biotic concentration. Whatever the antibiotic concentration, protoplas
t-derived cells kept significantly higher viability for at least 3 wee
ks compared with those cultured without antibiotic. As to cell wall re
generation after 2 d, it was not affected by the antibiotic except at
the highest concentration tested (100 mg l(-1)). Sporadic first cell d
ivision was observed after 2-6 d of culture in the presence of ciprofl
oxacin while, in its absence, cell division was never apparent before
10 d of culture. (C) 1995 Annal of Botany Compamy