AMPEROMETRIC ENZYME ELECTRODE FOR AEROBIC GLUCOSE MONITORING PREPAREDBY GLUCOSE-OXIDASE IMMOBILIZED IN POLY(VINYLFERROCENIUM)

A simple, sensitive, stable and low-cost glucose sensor with a fast re sponse that operates under aerobic solution conditions is described. T he electrode consists of a polyvinylferrocenium-perchlorate-coated Pt surface onto which the enzyme is attached. The current response of the electrode was determined by measuring either cyclic voltammetric peak current values or steady state current values obtained after a consta nt-potential step. The effects of glucose concentration, the amount of enzyme immobilized, the thickness of the polymeric layer and the oper ating temperature on the response of the enzyme electrode were studied . The effects of two common physiological sources of interference were also investigated. A mechanism for the operation of the electrode is also proposed.